TABLE 1.
In vitro stability of VapA expression in recombinant R. equi
| Passage | % Positive (MFI)a for VapA in strain:
|
|||
|---|---|---|---|---|
| 103+ | 103−/415 | 103−/415-VapAb
|
||
| With hygromycin | Without hygromycin | |||
| 2 | 88.7 (8.64) | 3.4 (1.26) | 85.9 (6.55) | 83.3 (6.78) |
| 3 | 86.2 (8.39) | 3.7 (1.19) | 84.8 (5.95) | 82.6 (5.52) |
| 4 | 85.4 (8.06) | 1.6 (1.18) | 85.2 (5.46) | 86.8 (5.58) |
| 5 | 83.2 (8.53) | 1.6 (1.19) | 86.3 (6.11) | 85.7 (5.40) |
a
Percentage of bacterial cells staining positively with MAb103 minus cells stained with an irrelevant control antibody. MFI values are given as the log mean fluorescence intensity of positively gated cells.
b
Bacterial samples were taken from cultures grown with or without 150 μg of hygromycin per ml.