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. 2024 Dec 18;24:1184. doi: 10.1186/s12870-024-05936-2

Fig. 10.

Fig. 10

Yeast two-hybrid assays of AeNHL17 protein. The pBT3-N-AeNHL17 and pOST1-NubI, pPR3-N were co-transformed into yeast NMY51 strains and cultured on DDO, TDO, QDO agar plates, respectively. The CDS of AeAKR was cloned into the prey vector pPR3-N and co-transformed with the bait vector pBT3-N-AeNHL17. Yeast cells co-transformed with pTSU2-App and pNubG-Fe65, pTSU2-App and pPR3-N were used as positive and negative controls, respectively