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. 2024 Dec 18;24:1184. doi: 10.1186/s12870-024-05936-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 7 — Overexpression of AeNHL17 enhances the disease resistance of tobacco. (a) Identify positive transgenic plants by using PCR analysis. (b) Relative expression of AeNHL17 in WT and transgenic tobacco lines. (c) Bacterial growth of Pseudomonas syringae pv. Actinidiae (Psa) and Pseudomonas syringae pv. Tomato DC3000 (Pst) in wild-type (WT) and transgenic lines (AeNHL17-4, AeNHL17-6, AeNHL17-11) at 3 days after inoculation. Bacterial growth is expressed as mean values of viable bacteria per square centimeter of leaf tissue. CFU: colony forming unit. (d) Disease symptom from representative leaves of WT and transgenic tobacco lines at 7 days after inoculation with Psa and Pst. Data were presented as mean values ± SD (n = 3). Lower-case letters in the column denote the significance level of mean differences at 0.05 (LSD method)