KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
ElectroMAX DH10B T1 Phage Resistant Cells	Invitrogen	12033015
BL21-AI E. coli	Invitrogen	C607003
Chemicals, peptides, and recombinant proteins
Agar	Fisher Scientific	BD214050
Kanamycin	Sigma	K-1377
Ampicillin	Sigma	A0166
Gibco bacto tryptone	Fisher Scientific	DF0123-17-3
Sodium Chloride	Fisher Scientific	S641-212
Gibco yeast extract	Fisher Scientific	DF0127-17-9
L-arabinose	Thermo Fisher	A11921.18
Paraformaldehyde	Electron Microscopy Sciences	15710
Coomassie brilliant blue	Gibco	15528–011
Methanol	Sigma	179337-2L
Acetic acid	Fisher Scientific	A38-212
Critical commercial assays
PicoPure RNA isolation kit	Applied Biosystems	KIT0204
Cloneminer II cDNA Library Construction Kit	Invitrogen	A11180
HiPure Plasmid Filter Midiprep Kit	Invitrogen	K210014
Purelink HQ Plasmid Purification Kit	Invitrogen	K2100-01
Kapa2G Robust Hotstart PCR Kit	Roche	07961073001
ChargeSwitch-Pro PCR cleanup kit	Invitrogen	CS32050
SuperScript III First-Strand Synthesis System	Invitrogen	18080–051
Q5 High-Fidelity 2x Master Mix	NEB	M0492
Gel DNA Recovery Kit	Zymo	D4002
NEBuilder HiFi Assembly Master Mix	NEB	E2621
Q5 Site-Directed Mutagenesis Kit	NEB	E0554
NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm	Thermo Fisher	NP0322BOX
Protein Assay Kit	Bio-Rad	5000002
TRIzol Max Bacterial RNA Kit	Invitrogen	16096020
BP clonase II	Invitrogen	11789–020
LR clonase II	Invitrogen	11791–020
Deposited data
Expression cloning sequencing data	This study	PRJNA1008366
Bacterial RNA-seq data	This study	PRJNA1070671
Experimental models: Organisms/strains
Hypsibius exemplaris (Z151)	Carolina Biological	133960
Ramazzottius c.f. varieornatus (YOKOZUNA-1)	Gift from K. Arakawa	N/A
Chlorococcum sp.	Carolina Biological	152091
Super Fresh Chlorella V12	Reed Mariculture	N/A
S. cerevisiae (W303) MATa ura3-1, leu2-3,112, his3-11, trp1-1, can1-100, ade2-1	American Tissue Culture Connection	208352
Oligonucleotides
cDNA insert amplification forward: CATCACCATCACCTCGAATCAAC	Integrated DNA Technologies	N/A
cDNA insert amplification reverse: TTCGGGCTTTGTTAGCAGCCTCGAATC	Integrated DNA Technologies	N/A
pDEST17 linearize forward: TGATTCGAGGCTGCTAACAAAG	Integrated DNA Technologies	N/A
pDEST17 linearize reverse: GTAGTACGACATATGTATATCTC	Integrated DNA Technologies	N/A
Recombinant DNA
pDONR222	Invitrogen	Included with A11180
pDEST17	Invitrogen	11803012
pDONR221	Invitrogen	12536017
pAG415	Addgene	Plasmid #14146
pFA6	Addgene	Plasmid #39296
M13mp18 ssDNA	NEB	N4040S
Software and algorithms
Bowtie2	https://bowtie-bio.sourceforge.net/bowtie2/index.shtml	N/A
Featurecounts	https://subread.sourceforge.net/featureCounts.html	N/A
DEseq2	https://bioconductor.org/packages/release/bioc/html/DESeq2.html	N/A
Integrative genomic viewer	https://igv.org/	N/A
Image Lab 5.2.1	Bio-Rad	N/A
FIJI	https://imagej.net/software/fiji/	N/A
EdgeR	https://bioconductor.org/packages/release/bioc/html/edgeR.html	N/A
RStudio	https://cran.r-project.org/https://posit.co/download/rstudio-desktop/#download	N/A
Jalview	https://www.jalview.org/download/	N/A
MEGAX	https://www.megasoftware.net/	N/A
Other
Vented 35 mm petri dishes	Tritech Research	T3500
Deer Park spring water	Staples	705032
Aspirator tube assembly	Millipore Sigma	A5177
Borosilicate glass capillary	World Precision Instruments	1B100F-4
Electroporator	Eppendorf	2510
S.O.C. media	Invitrogen	15544034
Speedvac	Savant
Drierite desiccant	Fisher Scientific	07-578-4A
Branson Sonifier 250	Branson	SFX250
Precision Plus Protein Kaleidoscope	Bio-Rad	1610375
MOPS SDS Running Buffer	Invitrogen	NP0001
Biotek Synergy H1 multimode reader	Biotek
Salmon sperm DNA	Agilent technologies	201190
Mitotracker Red FM	Invitrogen	M22425
DAPI Fluoromount-G	Southern Biotech	0100–20