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A) Tcj2 mRNA LNPs and empty LNPs were analyzed by Dynamic Light Scattering (DLS), directly after formulation and after storage at -80° C. Diameter in nm (Z-Ave (d.nm)) and polydispersity index (PdI) of LNPs were reported. B) Distribution of the size of the Tcj2 mRNA LNPs and empty LNPs. C) pKa of the surface of Tcj2 mRNA LNPs was analysed though a TNS fluorescence assay.
