Fig 7. Tcj2 mRNA LNP immunizations induced antigen-specific cytokine production in central memory and effector memory CD8+ T cells, as well as increase in cytokine producing polyfunctional CD8+ T cells.

A) antigen-specific central memory (CD62high and CD44high) and effector memory (CD62low and CD44high) CD8+ T cells were analyzed for production of granzyme B, perforin, IFN-γ or TNF-α. B) Antigen-specific CD8+ T cells were analyzed for the production of two or more cytolytic enzymes or cytokines using a Boolean combination gate strategy. For data presented in A and B: values from non-stimulated cells were subtracted from rTcj2 protein stimulated cells to obtain antigen-specific cytokine production. Statistical significance: *: p < 0.05, **: p < 0.01. For all panels in this figure the mean and standard deviation are shown. Filled symbol shapes represent in vivo study #1, while open symbol shapes represent repeat in vivo study #2.