Fig 8. Splenocytes from Tcj2 mRNA LNP—Immunized mice decrease T. cruzi infection in vitro.

T. cruzi–infected MC57G mouse fibroblasts were co-cultured with splenocytes from immunized mice for 72 hours. T. cruzi parasites expressed the LacZ gene which encodes for the enzyme β-galactosidase, used to convert a substrate which directly correlated to the parasite load in each well. T. cruzi–infected fibroblasts treated with a high dose of benznidazole were run in parallel and used to calculate the %-reduction of parasite load. Statistical significance: **: p < 0.0001. Mean and standard deviation are shown. Filled symbol shapes represent in vivo study #1, while open symbol shapes represent repeat in vivo study #2.