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. Author manuscript; available in PMC: 2024 Dec 18.
Published in final edited form as: Nat Methods. 2021 Dec 30;19(1):55–64. doi: 10.1038/s41592-021-01348-4

Extended Data Fig. 6 ∣. TAGA-qtRNA and AGGG-qtRNA PRANCE.

Extended Data Fig. 6 ∣

(a) Constructs for evolving TAGA-decoding qtRNAs. (b) Representative results for evolving TAGA-decoding qtRNAs. (c) Evolved qtRNAs exhibit increased ability to decode a TAGA quadruplet codon. Units are the percent luminescence when translating luxAB-357-TAGA in the presence of the qtRNA relative to expression of all-triplet-luxAB. (d) evolved genotypes E) Real-time absorbance and luminescence monitoring of qtRNA-encoding phage where either randomized or directed anticodons were used to evolve AGGG containing codons.