Figure 4.

The absence of the thymic stromal lymphopoietin (TSLP)/TSLP receptor (TSLPR) signaling pathway facilitates the infiltration of myeloid cells into cardiac tissue following myocardium infarction (MI). (A–C) The gating strategies employed in identifying CD45⁺FVD^- total CD45⁺ cells, CD45⁺FVD^-CD11b⁺Ly6G⁺ neutrophils, CD45⁺FVD^-CD11b⁺Ly6G^- monocytes/macrophages (Mono/Macro), CD45⁺FVD^-CD11b⁺Ly6G^-Ly6C^low monocytes, CD45⁺FVD^-CD11b⁺Ly6G^-Ly6C^hi monocytes, CD45⁺FVD^-CD11b⁺Ly6G^-F4/80⁺ macrophages, and CD45⁺FVD^-CD11c⁺MHC II⁺ dendritic cells in the heart. (D–K) Absolute numbers of the above cells per gram of cardiac tissue and the Ly6C^hi/Ly6C^low monocyte ratio were determined on day 3 and day 7 post-MI (n = 6–9 per group). Data are presented as the mean ± SEM and unpaired t-test was used to analyze in data (D–K).