Figure 7.

Validation of the detection of epilepsy-related phenotypes in a DIV14 set of the RHEB-p.P37L NDD model by the autoMEA software: (A) example raster plots with a 5-s section of a single electrode of a control (black, left) and RHEB-p.P37L (orange, right) well from manual MCS analysis and the spikes30 autoMEA model, and a raw data trace example of a single channel during a network burst for both genotypes at the bottom, black lines at the bottom represent spikes, orange bars (top) represent reverberations as detected with manual MCS analysis and the blue bars (middle) reverberations detected using the spikes30 autoMEA model. (B) Comparison of the network burst composition for control and RHEB-p.P37L cultures detected using all different burst detection methods. (C) Comparison of the network reverberation duration for control and RHEB-p.P37L cultures detected using all different burst detection methods. N = 11 wells/group. Student's t-test: ^***p < 0.0001, ^****p < 0.00001.