Figure 8.

Validation of the detection of epilepsy-related phenotypes in a DIV7 set of the RHEB-p.P37L NDD model by the autoMEA software: (A) example raster plots with a 5-s section of a single electrode of control (black, left) and RHEB-p.P37L (orange, right) well from manual MCS analysis and the signal100 autoMEA model, and a raw data trace example of a single channel during a network burst for both genotypes at the bottom, black lines at the bottom represent spikes, orange bars (top) represent reverberations as detected with manual MCS analysis and the blue bars (middle) reverberations detected using the signal100 autoMEA model. (B) Comparison of the %RS for control and RHEB-p.P37L cultures detected using all different burst detection methods. (C) Comparison of the network burst rate for control and RHEB-p.P37L cultures detected using all different burst detection methods. (D) Comparison of the NIBI for control and RHEB-p.P37L cultures detected using all different burst detection methods. (E) Comparison of the network burst duration for control and RHEB-p.P37L cultures detected using all different burst detection methods. N = 11 wells/group. Student's t-test: ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 ^***p < 0.0001.