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. 2024 Dec 18;15(12):890. doi: 10.1038/s41419-024-07283-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Uptake of NIR-715 by KYSE30 and KYSE150 cells, imaged by live-cell confocal fluorescence microscopy, at various times after NIR-715 addition. Fluorescence intensity was measured with a fluorescence microscope. Scale bar, 100 μm. B KYSE30 cells treated with vehicle (1% DMSO) or NIR-715 (2 μM) followed by light exposure (10 min) and SYTOX Green staining, as detected by live-cell confocal fluorescence microscopy. Scale bar, 100 μm. C Cell viability of KYSE30 (upper) and KYSE150 (lower) cells treated with various concentrations of NIR-715 with or without light exposure for 10 min. Cell viability was evaluated by MTS assay. D Colony formation assay for KYSE30 (upper) and KYSE150 (lower) cells after NIR-715 treatment. All data are presented as the mean ± SD (n = 4). **P < 0.01 ***P < 0.001.