Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Dec 18;10:498. doi: 10.1038/s41420-024-02260-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — A, B Ubiquitination assays of HEK-293T cells following expression of FLAG-GLI1, HA-UB and either control vectors, MYC-SMURF1 or MYC-SMURF2. 24 h after transfection, cells lysates were immunoprecipitated with FLAG agarose beads and analysed by SDS-PAGE. Proteins were detected using antibodies anti-FLAG, anti-MYC and specific antibodies against K48-linked/K63-linked poly-ubiquitination to detect the ubiquitination status of GLI1. C, D Analysis of GLI1 protein levels following SMURF1/SMURF2 expression in presence of proteasome inhibitor MG132. HEK-293T were transfected with HA-GLI1 or FLAG-GLI1, in combination with control vector, FLAG-SMURF1 or MYC-SMURF2. 24 h post transfection, cells were treated with DMSO or MG132 at 1 µM for 16 h. Subsequently, cells were lysed and analysed by SDS-PAGE. Proteins were detected using antibodies anti-FLAG, anti-HA, anti-MYC, and anti-VINCULIN (used as a normalizer).