Fig. 6. SMURFs expression reduces GLI1 dependent MB cell proliferation.

EdU incorporation staining on MB cells DAOY and ONS-76 expressing SMURF proteins (A–C) or stably transduced cells with the indicated shRNAs against SMURF1 or SMURF2 (B–D). Percentage of EdU positive cells was calculated over transfected or total cells as indicated. (*p < 0.05; **p < 0.01; ***p < 0.001 results are expressed as the mean ± SD of three independent experiments, Student’s t-test).