Fig. 1. Effects of drug-induced expression of GALNTL5 protein in GC-2spd(ts) cells.

A Western blotting with anti-GFP antibody. In the GC-2spd(ts) cell line with a chemically inducible expression vector for GALNTL5 tagged with GFP (GALNTL5-GFP/GC-2spd), GALNTL5 protein was detected as at least two bands for 3 days when the cumate chemical solution was present. B, C Fluorescence microscopy image of GALNTL5-GFP/GC-2spd cells after 24 h with or without chemical induction. White scale bars, 100 μm. D, E GALNTL5-GFP/GC-2spd cells at baseline and after 72 h, without chemical induction. Live-cell imaging of cell proliferation was performed with an All-in-one Fluorescence Microscope (Keyence). F, G Cell imaging of GALNTL5-GFP/GC-2spd at baseline and after 72 h of chemical induction. At 72 h there is continuous GALNTL5-GFP expression and shrinking cells are seen. White scale bars, 100 µm. Detailed cellular alterations with or without chemical induction are shown in the supplementary movies.