FIG. 2.

Growth of wild-type cells on the surface of rough or smooth agar. Cells of SC5314 (EFG1⁺/EFG1⁺ CPH1⁺/CPH1⁺) were plated on YPS plates containing rough and smooth areas and incubated at 25°C (see text). At various times, colonies were observed and photographed with a Nikon TM300 inverted-phase microscope with a 10× objective. The arrow in panel C indicates the edge of the colony. The arrow in panel D indicates filaments. Panels A and B show colonies grown on a smooth surface (A, 1 day; B, 3 days). Panels C and D show colonies grown on a rough surface (C, 1 day; D, 3 days).