FIG. 3.

Filamentous growth of strain CKY138. Exponentially growing cells of CKY101 (wild type) (A) and CKY138 (efg1/efg1 cph1/cph1) (B) grown in YPD medium (1% yeast extract, 2% Bacto Peptone, 2% glucose) at 30°C were mixed with molten YPS medium and plated. After 3 days of incubation at 25°C, colonies were photographed at a magnification of ×10. Panel C shows exponentially growing cells of strain CKY138 plated on a rough YPS agar surface, incubated at 25°C for 5 days, and photographed at a magnification of ×10. Panel D shows CKY138 cells extracted from a colony grown on rough YPS agar, stained with Calcofluor white, and photographed at a magnification of ×100 by using a standard 4′,6-diamidino-2-phenylindole (DAPI) filter set.