Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Dec 18;14(1):218. doi: 10.1038/s41408-024-01199-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

PMC Copyright notice

Fig. 2 — A UMAP visualization of various T/NK cell clusters (left) and subtypes (right). B Boxplot showing enrichment scores of AITL signature and stemness inferred by CytoTRACE across different T cells clusters. The boxplot represents the mean value and the error bar represents the standard error value. C Principal component analysis (PCA) of the clusters according to the mean transcription factor activity of each cluster. D Volcano plot of gene expression changes of Tfh cells between ND-AITL and RR-AITL. Upregulated (red) and downregulated (blue) genes in RR-AITL are highlighted. E Violin plot showing expression levels of CXCL13 in Tfh cells from ND-AITL and RR-AITL samples (upper panel). Violin plot showing enrichment score of AITL signatures in Tfh cells from ND-AITL and RR-AITL samples (bottom panel). P values were determined by two-sided unpaired Wilcoxon test. The boxplot represents the mean value and the error bar represents the standard error value. F GSEA plot showing the hallmarks that are significantly enriched in RR-AITL and ND-AITL, respectively. G Boxplot showing enrichment score of proliferation signature and PI3K/AKT pathway. P values were determined by two-sided unpaired Wilcoxon test. H Boxplot showing intensities of pAKT in IMC data. P values were determined by two-sided unpaired Wilcoxon test. I The IMC images showing the protein activity of pAKT within malignant Tfh cells. The colors represent protein activity levels, ranging from lower (blue) to higher (red). J, K Immunofluorescence analysis for ND-AITL and RR-AITL samples stained by an-ti-DAPI (blue), anti-CXCL13 (green), anti-pAKT (red) antibodies. Scale bar, 100 μm.