Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1975 Jul;149(1):23–30. doi: 10.1042/bj1490023

Structure and biosynthesis of the ribosomal ribonucleic acids from the oncogenic bacterium Agrobacterium tumefaciens.

J M Grienenberger, D Simon
PMCID: PMC1165588  PMID: 1191257

Abstract

The rRNA of the oncogenic bacterium Agrobacterium tumefaciens was extracted by several methods and analysed by polyacrylamide-gel electrophoresis. The large rRNA of this bacterium is degraded in vivo during the maturation of the ribosome. The influence of Mg2+ and denaturation on degradation of 23S RNA was studied. In pulse and chase experiments, we identified two precursors of the rRNA with mol.wts. of 1.04 x 10(6) and 0.70 x 10(6). From studies of the structure of the large rRNA, we propose that it could have arisen from a gene duplication. This structure is discussed in relation to a recent hypothesis involving such gene duplication as a means of origin of 23S rRNA.

Full text

PDF
23

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adesnik M., Levinthal C. Synthesis and maturation of ribosomal RNA in Escherichia coli. J Mol Biol. 1969 Dec 14;46(2):281–303. doi: 10.1016/0022-2836(69)90422-7. [DOI] [PubMed] [Google Scholar]
  2. Applebaum S. W., Ebstein R. P., Wyatt G. R. Dissociation of ribonucleic acid from silkmoth pupae by heat and dimethylsulfoxide: evidence for specific cleavage points. J Mol Biol. 1966 Oct 28;21(1):29–41. doi: 10.1016/0022-2836(66)90077-5. [DOI] [PubMed] [Google Scholar]
  3. Braun A. C., Wood H. N. On the inhibition of tumor inception in the crown-gall disease with the use of ribonuclease A. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1417–1422. doi: 10.1073/pnas.56.5.1417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Click R. E., Hackett D. P. The isolation of ribonucleic acid from plant, bacterial or animal cells. Biochim Biophys Acta. 1966 Oct 24;129(1):74–84. doi: 10.1016/0005-2787(66)90010-4. [DOI] [PubMed] [Google Scholar]
  5. Greenberg J. R. Synthesis and properties of ribosomal RNA in Drosophila. J Mol Biol. 1969 Nov 28;46(1):85–98. doi: 10.1016/0022-2836(69)90059-x. [DOI] [PubMed] [Google Scholar]
  6. Grienenberger J. M., Simon D. Etude de la structure et de la dégradadation des acides ribonucléiques ribosomaux de quelques bactéries du genre Agrobacterium. C R Acad Sci Hebd Seances Acad Sci D. 1972 Feb 28;274(9):1399–1402. [PubMed] [Google Scholar]
  7. Hecht N. B., Woese C. R. Separation of bacterial ribosomal ribonucleic acid from its macromolecular precursors by polyacrylamide gel electrophoresis. J Bacteriol. 1968 Mar;95(3):986–990. doi: 10.1128/jb.95.3.986-990.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Higo S., Higo M., Tanifuji S. Specific dissociation of pea 25-S rRNA by hot-phenol treatment. Biochim Biophys Acta. 1971 Sep 24;246(3):499–506. doi: 10.1016/0005-2787(71)90787-8. [DOI] [PubMed] [Google Scholar]
  9. Ingle J., Possingham J. V., Wells R., Leaver C. J., Loening U. E. The properties of chloroplast ribosomal-RNA. Symp Soc Exp Biol. 1970;24:303–325. [PubMed] [Google Scholar]
  10. KIRBY K. S. ISOLATION AND CHARACTERIZATION OF RIBOSOMAL RIBONUCLEIC ACID. Biochem J. 1965 Jul;96:266–269. doi: 10.1042/bj0960266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Koser R. B., Collier J. R. The molecular weight and thermolability of Ilyanassa ribosomal RNA. Biochim Biophys Acta. 1971 Dec 16;254(2):272–277. doi: 10.1016/0005-2787(71)90836-7. [DOI] [PubMed] [Google Scholar]
  12. Le Goff L. Acides ribonucléiques des bactéries du crown-gall Agrobacterium tumefaciens (Smith et Town) Conn. exposées au borate de sodium. Ann Inst Pasteur (Paris) 1968 Aug;115(2):232–248. [PubMed] [Google Scholar]
  13. Leaver C. J., Ingle J. The molecular integrity of chloroplast ribosomal ribonucleic acid. Biochem J. 1971 Jun;123(2):235–243. doi: 10.1042/bj1230235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Loening U. E., Ingle J. Diversity of RNA components in green plant tissues. Nature. 1967 Jul 22;215(5099):363–367. doi: 10.1038/215363a0. [DOI] [PubMed] [Google Scholar]
  15. Loening U. E. Molecular weights of ribosomal RNA in relation to evolution. J Mol Biol. 1968 Dec;38(3):355–365. doi: 10.1016/0022-2836(68)90391-4. [DOI] [PubMed] [Google Scholar]
  16. Loening U. E. Ribosomal ribonucleic acid in evolution. Biochem Soc Symp. 1973;37(0):95–104. [PubMed] [Google Scholar]
  17. Loening U. E. The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation. Biochem J. 1969 Jun;113(1):131–138. doi: 10.1042/bj1130131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Marrs B., Kaplan S. 23 s precursor ribosomal RNA of Rhodopseudomonas spheroides. J Mol Biol. 1970 Apr 28;49(2):297–317. doi: 10.1016/0022-2836(70)90247-0. [DOI] [PubMed] [Google Scholar]
  20. Nomura M. Bacterial ribosome. Bacteriol Rev. 1970 Sep;34(3):228–277. doi: 10.1128/br.34.3.228-277.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rawson J. R., Crouse E. J., Stutz E. The integrity of the 25-S ribosomal RNA from Euglena gracilis 87-S ribosomes. Biochim Biophys Acta. 1971 Sep 24;246(3):507–516. doi: 10.1016/0005-2787(71)90788-x. [DOI] [PubMed] [Google Scholar]
  22. Solymosy F., Fedorcsák I., Gulyás A., Farkas G. L., Ehrenberg L. A new method based on the use of diethyl pyrocarbonate as a nuclease inhibitor for the extraction of undegraded nucleic acid from plant tissues. Eur J Biochem. 1968 Sep 24;5(4):520–527. doi: 10.1111/j.1432-1033.1968.tb00401.x. [DOI] [PubMed] [Google Scholar]
  23. Stanley W. M., Jr, Bock R. M. Isolation and physical properties of the ribosomal ribonucleic acid of Escherichia coli. Biochemistry. 1965 Jul;4(7):1302–1311. doi: 10.1021/bi00883a014. [DOI] [PubMed] [Google Scholar]
  24. Stevens A. R., Pachler P. F. Discontinuity of 26 s rRNA in Acanthamoeba castellani. J Mol Biol. 1972 May 14;66(2):225–237. doi: 10.1016/0022-2836(72)90475-5. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES