Fig. 9.

Classification of the analyzed Flower isoforms and mutants into rescuer, partial- and non-rescuer. A Bar graph with all analyzed Flower isoforms and mutants sorted by the normalized delta values calculated from time points 0 min and 15 min. Rescuers show > 80% rescue, partial rescuers 30–60% and non-rescuers < 30% endocytosis rescue. Data given as mean ± SEM. B The secondary structure of the full-length mouse Fwe2 protein with transmembrane domains (TMs) predicted by the CCTOP prediction tool, similar to the previously postulated topology (Yao et al., 2017, Chang et al., 2018). The mutations and the putative selectivity pore are drawn in red and gray, and the minimal region necessary for endocytosis rescue is drawn in the gray box according to (A)