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. 2024 Dec 18;15(12):905. doi: 10.1038/s41419-024-07285-2

Fig. 6. Growth behavior in vitro and expression of different markers of the newly established RB derived stromal cell line T18 as revealed by immunohistochemical stains.

Fig. 6

a Paraffin sections of RB tumor tissue (T18 tumor), primary RB derived stromal cells (T18) and the respective immortalized RB derived stromal cells (T18_IM) stained for different markers (brown signal) with hematoxylin and eosin counterstaining (upper row) or after immunofluorescence staining for markers (red fluorescence) and DAPI counterstaining (blue fluorescence; middle and lower row; scale bars: 100 µm). b Growth curve analysis of the immortalized T18_IM RB stromal cells showing mean doubling times of 36 h. Immortalized T18 stromal cells (T18_IM) showed significantly increased cell viability and proliferation levels compared to primary T18 stromal cells (T18) as revealed by (c) WST-1 assay and (d) BrdU stains. Values are means of three independent experiments ± SEM. ****p < 0.0001 statistical differences compared to the control group calculated by Student’s t-test.