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. 2024 Dec 18;11:1372. doi: 10.1038/s41597-024-04252-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 1 — Reference accessions of Abeliophyllum distichum and Forsythia ovata used in this study. (a) Photographs of A. distichum (left) and F. ovata (right) flowers, fruits, and trees, taken between March and April 2023. (b) Estimation of genome sizes for A. distichum (Ad) and F. ovata (Fo) based on K-mer 25 distributions. The blue and red peaks correspond to heterozygous and homozygous reads, respectively. The lower panel depicts the estimated haploid genome size derived from homozygous K-mer peaks and flow cytometry analyses. (c) Workflow of genome assembly and annotation.