Fig. 5. Calcium uptake studies in accutase-treated 2D-cultured cells on day 7 of differentiation.

A Calcium-uptake activities of WT and Jdp2 KO Gabra6⁺ GCPs. Inositol triphosphate (IP3)-mediated Ca²⁺ liberation was assessed in neuronal cells using a two-beam-signal cell simulation system. The IP3 (0.5 μM) stimulation ΔF/F ratio and the calcium-uptake activities were evoked to a higher degree in Jdp2 KO GCPs after IP3 uncaging (n = 5; *P < 0.05). B A Gaba receptor agonist (GABOB) (100 μM) stimulated a greater release of Ca²⁺ in Jdp2 KO Gabra6⁺ GCPs (1.34^-fold higher compared with WT GCPs) (n = 5; ***P < 0.001). C The effects of Gaba receptor inhibitors, i.e., bicuculline (10 μM), PTZ (100 μM), and flumazenil (10 μM), on GABOB stimulated the release of Ca²⁺ in WT and Jdp2 KO Gabra6⁺ GCPs. The protocol used for the liberation of Ca²⁺ was as described in the Materials and Methods (n = 5; *P < 0.05; **P < 0.01; ***P < 0.001).