FIG. 1.

Effect of CNF1-containing bacterial extracts on repair of wounded T24 (A and B) or Hs 738 (C and D) bladder cell monolayers. Confluent monolayers in 60-mm-diameter dishes were wounded and incubated with medium containing CNF1 (DH5/pISS392) or control (DH5/pGEM3) bacterial extracts for 24 h. The plates were fixed and stained with Giemsa stain. The mean number of nuclei entering the wounded region and the mean maximum distance the cells migrated were measured and expressed as percentages of the values obtained with the control wounds (incubated in medium only). Percentages of the number of cell nuclei (A and C) and maximum distance migrated by cells (B and D) in control wounds are the means ± SEM of four (T24 cells) or six (Hs 738 cells) experiments.