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. 2024 Dec 3;9(50):49503–49512. doi: 10.1021/acsomega.4c07249

Figure 3.

Figure 3

Verification of the NM-RT-MCDA assay. (A) The NM-RT-MCDAassay was performed at 65 °C for 40 min using real-time fluorescence detector monitoring the results. (B) For primer verification, NM-MCDA assay was carried out at 65 °C for 40 min and the results were reported using PCR turbidimeter. (C) Amplification products of the NM-MCDA assay were visually analyzed by observation of the color change. (D) A lateral flow biosensor was applied for visual detection of NM-MCDA products. NM: N. meningitis, PC:positive control, NC: negative control, BC: blank control,TL: testing line, CL: control line.