FIG. 2.

Induction of iNOS protein synthesis of human monocytic cells by PMA stimulation and Salmonella infection. The expression of iNOS protein in U937 cells (2 × 10⁶) transfected with either DNA containing the gene for HLA-A2 (U937+A2) or HLA-B27 (U937+B27g) or cDNA encoding HLA-B27 (U937+B27c) was studied by Western blotting. Mouse macrophages (J774.A1) (10⁶) were used as a positive control after stimulation with IFN-γ and LPS. J774.A1 cells without any stimulation and a subclass-matched control MAb, KIT-2C75, were used as negative controls.