. 2024 Dec 4;15:1460729. doi: 10.3389/fmicb.2024.1460729

Table 2.

Primer information for common RT-qPCR detection of soil borne pathogens.

Disease	Pathogen	Primer sequence (5′-3′)	Target gene	Fragment size (bp)	References
Root rot	Fusarium sp.	F8-1:GCTTCTCCCGAGTCCCA	EF-1α	187	Chen et al. (2019)
	Fusarium sp.	F8-2:GCTCAGCGGCTTCCTAT		187	Chen et al. (2019)
	F. oxysporum	18F:TAATGCTCGTAAGTCAGGTCAGGTCAGGTTCA		172	Zhong et al. (2022)
	F. oxysporum	18R:AGTTGGAGTCAGCGATTCAT		172	Zhong et al. (2022)
	Aphanomyces cochlioides	AcF:TCCGGGCTAGCCGAAGGTT	rRNA	96	Almquist et al. (2016)
	Aphanomyces cochlioides	AcR:ACAAGCAATCATTTCTGATGCTAGATAG	rRNA	96	Almquist et al. (2016)
Sheath blight	Rhizoctonia solani	AG-F:CACCTTTTGCTCTTTTTTTAATCCA	ITS	150	Vojvodic et al. (2019)
Sheath blight	Rhizoctonia solani	AG-R:ATAAATTGGGTTTATATTAGAGTTGAGTAGACA	ITS	150	Vojvodic et al. (2019)
Gummy stem blight	Stagonosporopsis spp.	DBF1:TCGAATGGCTCAGAGAAGGT	RGI	208	Murolo et al. (2022)
Gummy stem blight	Stagonosporopsis spp.	DBR1:AAGTCCACGTCAGACCCATC	RGI	208	Murolo et al. (2022)
Sclerotinia rot	Sclerotinia sp.	Scl SF:CTCAAATCTCCGAAAGTT	β-tubulin	237	Li et al. (2011)
Sclerotinia rot	Sclerotinia sp.	Scl AF:TGCAGACGGGTAATATG		237	Li et al. (2011)
Verticillium wilt	Verticillium dahliae	VertBt-F:AACAACAGTCCGATGGATAATTC		115	Duressa et al. (2011)
Verticillium wilt	Verticillium dahliae	VertBt-R:GTACCGGGCTCGAGATCG		115	Duressa et al. (2011)
Anthracnose	Colletotrichum spp.	cuti-F:AGAACCAGATCAAGGGCGTCGTG	Cutinase	222	Tavernier and Coenye (2015)
Anthracnose	Colletotrichum spp.	cuti-R:GCGTCCGCAATGTCGCAGTA	Cutinase	222	Tavernier and Coenye (2015)
Phytophthora blight	Phytophthora capsici	YM2F:ATTCCTCCTGATAGATAG	Actin	245	Nocker et al. (2006)
Phytophthora blight	Phytophthora capsici	YM2R:CCCTCATCACAGAATGC	Actin	245	Nocker et al. (2006)
Late blight	Phytophthora infestans	qPCR F:CATCGGTGTTGACTTTGTG	Ypt1	203	Khan et al. (2017)
Late blight	Phytophthora infestans	qPCRR:TGAGCAATGTAATGGCAATC	Ypt1	203	Khan et al. (2017)
Bacterial wilt	Ralstonia solanacearum	OLI-1:GGGGGTAGCTTGCTACCTGCC	16S rRNA	288	Ramesh et al. (2011)
Bacterial wilt	Ralstonia solanacearum	Y2: CCCACTGCTGCCTCCCTAGGAGT		288	Ramesh et al. (2011)
Bacterial fruit blotch	Acidovorax citrulli	Acf3: CCTCCACCAACCAATACGCT		550	Zhao et al. (2009)
Bacterial fruit blotch	Acidovorax citrulli	Aacr2: TCGTCATTACTGAATTTCAACA		550	Zhao et al. (2009)
Bacterial canker	Clavibacter michiganensis sub sp. michiganensis	Cmm141F: CAGGCGTCCGTCGGTGAGGTGGTC	pat-1	141	Cho et al. (2012)
Bacterial canker	Clavibacter michiganensis sub sp. michiganensis	Cmm141R: GCGGGAGAGCGGTGCGGGAATG	pat-1	141	Cho et al. (2012)