Table 3.
Primer information for common PMA-qPCR detection of soil borne pathogens.
| Disease | Primer sequence (5′-3′) | PMA concentration | Light time | Target gene | Fragment size (bp) | References |
|---|---|---|---|---|---|---|
| Root rot (Fusarium sp.) | F8-1:GCTTCTCCCGAGTCCCA | 50 mmol·L−1 | 15 min | EF-1α | 187 | Chen L. et al. (2022) |
| F8-2:GCTCAGCGGCTTCCTAT | ||||||
| Early blight (Alternaria spp.) | Alt4:CTTTTGCGTACTTCTTGTTTCC | 65 μmol·L−1 | 10 min | ITSs | 240 | Crespo-Sempere et al. (2013) |
| Alt5:CAGGCATGCCCTTTGGATAC | ||||||
| Corynespora blight (Corynespora cassiicola) | CC-F3:CAGGAAATCCTCGCCAAGCAG | 80 μmol·L−1 | 10 min | SdhB | 109 | Xie X. et al. (2022) |
| CC-R3:CGCCAGTGATACGGTTGAACGG | ||||||
| Clubroot (Plasmodiophora brassicae) | PBF3:TCTTGCGTGTCGCTGTATTC | 4 μmol·L−1 | 10 min | 16S rDNA | ~150 | Li et al. (2022) |
| PBR3:ATAGGTTGGGGTAACTTGGC | ||||||
| Bacterial speck (Pseudomonas syringae) | Pst3F:GCTGCGGATGGCAAGTC | 10 μmol·L−1 | 10 min | HrpZ | 161 | Chai et al. (2020) |
| Pst3R:CCGACACCCGAACCAGAAC | ||||||
| Bacterial wilt (Ralstonia solanacearum) | RS72F:ATGGATAAAGGGTTCGTGGTG | 3 ng·μL−1 | 5 min | 16S rDNA | 241 | Cao et al. (2015b) |
| RS312R:CAGGCTCAGCGAGATTGC | ||||||
| Bacterial fruit blotch (Acidovorax citrulli) | FP:CTGATAATCCTCGGCTCAACAA | 3 μg·mL−1 | 5 min | ArgA | 121 | Tian et al. (2016) |
| RP:TGAGCGCATTTCTGACGAG | ||||||
| Cucumber angular leaf spot (Pseudomonas syringae pv. lachrymans) | Pslgap1-F:TCGGCGACGCAATCAAT | 60 μmol·L−1 | 10 min | Gap1 | 162 | Meng et al. (2016) |
| Pslgap1-R:GGTGGTTTCACGCTTCAGG | ||||||
| Bacterial canker (Clavibacter michiganensis sub sp. michiganensis) | Spm4f:TCAGGCGTCTGTTCTGGC | 5 μmol·L−1 | 30 min | ITS | ~150 | Luo et al. (2008) |
| Spm2r:CCCACCACCATCCACAAC | ||||||
| Black spot (Pseudomonas syringae) | PM1:GCGAAGCGACAQCAACAGTG | 10 μg·mL−1 | 10 min | FliC | ~150 | Yu et al. (2021) |
| PM3:CGAGTCGATAGCGGCAAC |