TABLE 1.

Survival at 1 day post‐fertilization (dpf) and frequencies of positivity for mutation at 3 dpf observed in embryos injected with the Cas9 protein and a tyrosinase guide RNA. Results of four independent trials are shown.

	Survival rate (1 dpf)	Survival rate (3 dpf)	Positive mutation rate (3 dpf)
Trial 1	11/27 (41%)	8/27 (30%)	8/8 (100%)
Trial 2	16/29 (55%)	10/29 (34%)	6/10 (60%)
Trial 3	34/60 (57%)	29/60 (48%)	13/29 (45%)
Trial 4	12/20 (60%)	11/20 (55%)	11/11 (100%)

Note: Survival rate (%) = Surviving embryos (1 or 3 dpf)/Injected eggs. Positive mutation rate (%) = Positive mutants (3 dpf)/Surviving embryos (3 dpf).