Table 1. Kinetic Rate Constants for No-PDE and Sb-PDEa.
|
No-PDE |
Sb-PDE |
|||||
|---|---|---|---|---|---|---|
| compound | kcat (s–1) | Km (mM) | kcat/Km (M–1 s–1) | kcat (s–1) | Km (mM) | kcat/Km (M–1 s–1) |
| diesters | ||||||
| 1 | 6.7 | 0.62 | 1.1 × 104 | 19.8 | 0.14 | 1.4 × 105 |
| 2 | 49 | 2.3 | 2.1 × 104 | 49 | 0.9 | 5.0 × 104 |
| 3 | nd | nd | 7.7 × 102 | nd | nd | 3.9 × 101 |
| 4 | nd | nd | 1.3 × 104 | nd | nd | 2.8 × 103 |
| 5 | nd | nd | 4.1 × 103 | nd | nd | 3.4 × 103 |
| 6 | nd | nd | 1.4 × 103 | nd | nd | 7.0 × 102 |
| 7 | nd | nd | 1.2 × 102 | nd | nd | 3.5 × 101 |
| 8 | NO | NO | <1 × 100 | NO | NO | <1 × 100 |
| 9 | NO | NO | <1 × 100 | NO | NO | <1 × 100 |
| 10 | nd | nd | 6.4 × 102 | nd | nd | 8 × 102 |
| monoesters | ||||||
| 11 | 0.7 | 1.4 | 5.0 × 102 | 0.63 | 0.14 | 4.4 × 103 |
| 12 | nd | nd | 3.3 × 101 | 0.21 | 1.4 | 1.6 × 102 |
| 14 | nd | nd | <1 × 101 | NO | NO | <1 × 100 |
| 17 | nd | nd | <5 × 100 | NO | NO | <1 × 100 |
a
nd = not determined. NO = reaction not observed. Experimental errors were generally less than 10% and are given in the SI.