Fig. 5.

STING stabilizes TKT by inhibiting UBE3A-mediated TKT ubiquitination. A Pan-phosphorylation antibody of serine and threonine was used to detect TKT phosphorylation. B, C Identification of K48-linked ubiquitination of TKT in 293 T cells transfected with His-Ub and treated with MG132. D, E STING downregulates K48-linked ubiquitination of TKT. F, G Detection of interactions among STING, TKT, and UBE3A using specific antibodies. H Detection of the correlation between STING and UBE3A by western blots in KYSE-30 cells with STING knockdown and overexpression. I Effect of UBE3A on the ubiquitination of TKT was detected by immunoprecipitation in KYSE-30 cells transfected with His-Ub and shUBE3A. J K48-linked ubiquitination of TKT was detected by immunoprecipitation in STING-knocked down KYSE-30 cells transfected with His-Ub and shUBE3A. Data are mean ± SEM from three biological replicates