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. 2024 Dec 18;19(1):1–14. doi: 10.1080/19336918.2024.2442349

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© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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AltText: knockdown HGH1 inhibited the invasion and migration of BC cells. — Effects of HGH1 knockdown in BC cells. (a) HGH1 protein in MCF-10A, MDA-MB-231 and MCF-7. (b) Quantitative analysis of western blot. (c) Fluorescence map of HGH1 knockdown cell construction. (d) MDA-MB-231 and MCF-7 were transfected with shHGH1, and HGH1 was assessed using qRT-PCR. (e, f) Cell proliferation was measured through CCK-8 assays, and (g, i) colony formation was evaluated. (h, j) Invasion assays were performed to assess cell migration, while (k–n) wound-healing assays were performed to assess the metastatic potential. (o) ER protein expression. (p) Quantitative analysis of western blot. “*, p < .05; **, p < .01, ***, p < .001”.