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. 2024 Dec 19;43:326. doi: 10.1186/s13046-024-03245-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — scRNA-seq reveals five subsets of macrophages. A UMAP of macrophage clusters colored according to inferred cell type (left) and group (right). B Heatmap of the DEGs corresponding to macrophage clusters. C Fractions of macrophage clusters detected in CIM, IIM and EGC samples. D Heatmap of the macrophage-related gene signature scores in macrophage subsets. E Bubble plot of macrophage tissue-resident genes among macrophage clusters. F Spearman’s rank correlation between FOLR2 gene expression and macrophage tissue-resident gene expression in the TCGA-STAD cohort. G Representative mIHC images of CD68, FOLR2 and CD163 in CIM tissues. Scale bar, 25 μm. H Representative mIHC images of FOLR2 and CD31 in CIM tissues. Scale bar, 25 μm