Fig. 5.

FOLR2⁺ macrophages are positively correlated with CD8⁺ T cells during EGC carcinogenesis. A The proportions of each T-cell cluster from our scRNA-seq data. B Representative immunohistochemistry images of CD8A in CIM, IIM and EGC tissues (n=8). Scale bar, 50 μm. C Pearson correlation between FOLR2⁺ macrophages and CD8⁺Teff proportions in CIM, IIM and EGC tissues from our scRNA-seq data. D Representative mIHC images of CD68, FOLR2 and CD8 in CIM, IIM and EGC tissues. Scale bar, 100 μm. The Pearson correlation between the proportions of FOLR2⁺macrophages and CD8⁺Teff cells in CIM, IIM and EGC tissues was analyzed. E Spearman correlation between FOLR2 and CD8A mRNA expression in healthy control, CIM, IIM and EGC tissues from GSE78523. F Spearman correlation between FOLR2 and CD8A mRNA expression and paracancerous normal tissues from the TCGA STAD database. G Representative mIHC images of CD8A and FOLR2 expression in NAG tissue. Scale bar, 50 μm. H Representative flow cytometry plots of IFN-γ expression in CD8⁺T cells cocultured with FOLR2⁺ macrophages and different epithelial cell supernatants (n=3). I Representative flow cytometry plots of TNF-α expression in CD8⁺T cells cocultured with FOLR2⁺ macrophages and different epithelial cell supernatants (n=3). The data were analyzed by Pearson correlation analysis, Spearman correlation analysis or the Kruskal‒Wallis test. *p < 0.05; **p < 0.01; ***p < 10⁻³; ****p < 10⁻⁴; NS: not significant