Figure 1.

Induction of tissue resident memory T cells in mice after a single MV Schwarz immunization. hCD46⁺/IFNAR^−/− mice (n = 4–6 per group) were immunized by IP route with 10⁵ TCID₅₀ of MV Schwarz or were mock-injected (opti-MEM mediumMEM). Twenty-one days post-immunization, lymphocytes were extracted from organs (liver, lungs, and spleen) and surface markers were stained to identify the different subpopulations. (a) Percentage and absolute number of CD8⁺ T cells, resident memory CD8⁺ T cells (CD8⁺ Trm), measles-specific Trm (TrmMV), CD3⁺CD8⁻ T cells, and resident memory CD3⁺CD8⁻ T cells in the liver. (b) Percentage and absolute number of CD8⁺ T cells, resident memory CD8⁺ T cells (CD8⁺ Trm), measles-specific trm (TrmMV), CD4⁺ T cells, and resident memory CD4⁺ T cells (CD4⁺ Trm in the lungs. (c) Representative flow cytometry plots demonstrating the detection of liver mv-specific dextramer H_22–30 (gated on CD8⁺ CD69⁺ KLRG1⁻ CD62L⁻ CD11a⁺ CXCR6⁺ CXCR3⁺) on control or MV Schwarz immunized mice. (d) Representative flow cytometry plots demonstrating the detection of lung mv-specific dextramer H_22–30 (gated on CD8⁺ CD44⁺ CD62L⁻ CD69⁺ CXCR3⁺ CD49⁺) on control or MV Schwarz immunized mice. (e) Representative flow cytometry plots showing the specific lung and liver markers used to define tissue-resident T cell populations. Each data point represents an individual mouse. Data are compiled from two experiments and are represented as median with IQR (interquartile range). Significant differences between the groups were determined by the two-tailed Mann–Whitney test (*p < .05, **p < .01, ***p < .001).