Figure 3.

Cytokine expression profile of CD8⁺ Trm cells. (a) Representative flow plots and graphs showing the CD8⁺ Trm population (gated on CD8⁺ CD44⁺ CD62L⁻ CD69⁺ CXCR3⁺) and the absolute number of CD8⁺ Trm cells/lung in MV Schwarz versus control group. (b) Representative flow plots and graphs showing the MV-specific CD8⁺ Trm population (gated on CD8⁺ CD44⁺ CD62L⁻ CD69⁺ CXCR3⁺ CD49⁺ CD103^±) and the absolute number of MV-specific Trm cells/lung in MV Schwarz versus control group. (c) Total count of TrmMV/lung producing at least one or more cytokines, total count of TrmMV/lung producing GrzB, IFN-γ or TNF-⍺. Each data point represents an individual mouse. Data are compiled from one independent ICS experiment at early time point (d21 post-immunization). Data are represented as mean with standard error. Significant differences between the groups were determined by the two-tailed Mann–Whitney test (*p < .05, **p < .01, ***p < .001). (d) Proportion of TrmMV producing only one cytokine or a combination of different cytokines in MV Schwarz immunized group (total and individual mice graphs).