Figure 4.

Cytokine expression profile of CD4⁺ cells. (a) Total count of CD4⁺ CD69⁺ T cells, CD4⁺ CD69⁺ T cells expressing at least one cytokine, CD4⁺ CD69⁺ T cells expressing IFN-γ or TNF-⍺ per lung in MV Schwarz versus control group. Each data point represents an individual mouse. Data are compiled from one independent ICS experiment at early time point (d21 post-immunization). Data are represented as mean with standard error. Significant differences between the groups were determined by the two-tailed Mann–Whitney test (*p < .05, **p < .01, ***p < .001). (b) Proportion of CD4⁺ CD69⁺ T cells producing one cytokine or a combination of two cytokines in MV Schwarz immunized group (total and individual mice graphs).