Figure 5.

Longevity and functionality of Trm cells 90 days after a single immunization. (a) Absolute number of CD8⁺ Trm cells/lung and mv-specific CD8⁺ Trm cells/lung in immunized MV Schwarz group at 21 days p.I., 90 days p.I, or control group. (b) Total count of CD8+ Trm cells/lung producing GrzB, IFN-γ or TNF-⍺ in IN versus IM MV Schwarz immunized group. (c) Proportion of CD8⁺ Trm producing only one cytokine or a combination of different cytokines in IN and IM MV Schwarz immunized group. (d) Total count of CD4⁺ CD69⁺ T cells/lung expressing IFN-γ or TNF-⍺ in IN versus IM MV Schwarz immunized group. (e) Proportion of CD4⁺ CD69⁺ T cells producing one cytokine or a combination of two cytokines in IN and IM MV Schwarz immunized group. Each data point represents an individual mouse. Data at d21 in (a) are derived from the ICS experiment at early time point in Figure 3ab. Data at d90 (bcde) are derived from one independent ICS experiment at late time point. Data are represented as mean with standard error. Significant differences between the groups were determined by the two-tailed Mann–Whitney test (*p < .05, **p < .01, ***p < .001).