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Ultra-performance liquid chromatography solvent gradient conditions for the separation of polyamines.
| Time (min) | Solvent A1 (%) | Solvent B2 (%) |
|---|---|---|
| 0 | 90.0 | 10.0 |
| 0.50 | 90.0 | 10.0 |
| 8.00 | 0.0 | 100.0 |
| 9.00 | 0.0 | 100.0 |
| 9.50 | 90.0 | 10.0 |
| 11.00 | 90.0 | 10.0 |
Solvent A: 0.1% formic acid in water.
Solvent B: 0.1% formic acid in acetonitrile.
