Fig. 3.

Characterization and performance of the reversible BiCAR-T cells. A Scheme of the reversible BiCAR device in primary T cells. B Western blot assays showed that BOCK could induce the BiCAR system in a concentration and time dependent manner. C Western blot assays showed the reversibility of BiCAR device-mediated CAR expression in primary T cells. BOCK was added on Day1–3 and Day6–8, while it was withdrawn on Day4–5 and Day9–10. D qPCR assay revealed that the mRNA level of CAR in the BiCAR device was stable regardless of the presence of BOCK. E The release of cytokines including TNF-α and GZMB, and the CD107a degranulation of BiCAR-T cells with BOCK at different concentrations when they were cocultured with Nalm6 cells for 24 h. F The cytotoxicity of the BiCAR-primary T cells cocultured with Nalm6-Luc cells and BOCK for 48 h at E/T ratios (2:1, 1:1, and 1:2). The data are reported as the means ± SDs (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, not significant (ns) by t tests