Fig. 5.

Anti-tumor capability of BiCAR-T cells in mice. A Body weights of mice that were injected with BOCK every two days for one month. The data are reported as the means ± SDs (n = 6). B BiCAR-T cells were injected intraperitoneally, followed by BOCK injection. After 24 h, the peritoneal cells were collected and stained for CAR protein detection via flow cytometry. C The experimental design for assessing the anti-tumor efficacy of BOCK induced BiCAR system in xenograft leukemia mouse model. The pattern was created with BioGDP.com [58]. D Analysis of tumor burden progression in mice via bioluminescence imaging every 7 days. E The survival status of the mice in the different groups. F Quantification analyzation of the tumor burden by the average luminescence. *p < 0.05, **p < 0.01, ***p < 0.001, not significant (ns) by t or Mantel-Cox tests