Fig. 6.

Characterization and cytotoxicity of the BiCAR system in NK cells. A Western blot assay showed that the BOCK could induce BiCAR system in NK92 cells. B The cytotoxicity of BiCAR-NK cells cocultured with Nalm6-Luc cells and BOCK for 24 h at E/T ratios (4:1, 2:1, 1:1, 1:2, and 1:4). The data are reported as the means ± SDs (n = 3). C Flow cytometry analysis of CD107a degranulation, and NKG2D, CD69, and IFN-γ expression in BiCAR-NK cells with BOCK in different concentration when they were cocultured with Nalm6-Luc for 6 h. D Analysis of tumor burden progression in mice by bioluminescence imaging every 7 days (n = 5). E Quantification analyzation of tumor burden by the average luminescence. F The survival status of mice in the different groups. G Schematic showing the BiCAR device design and mechanism. *p < 0.05, **p < 0.01, ***p < 0.001, not significant (ns) by t or Mantel-Cox tests