Fig. 1.

Comparison of read length and quality (Phred scale) between PacBio HiFi and ONT Duplex reads. A Distribution of read length vs quality of HiFi and Duplex reads with vertical dotted lines indicating the average lengths: 17 kbp for HiFi and 29.5 kbp for Duplex reads. On average, more than 50% of both Duplex and HiFi reads have quality scores ≥ Q30, a general cutoff for high-quality reads, indicated by the horizontal dotted line. B Comparison of read quality among ONT Simplex, ONT Duplex, and PacBio HiFi, with vertical dotted lines representing average quality scores: Q16 for Simplex, Q29 for Duplex, and Q32 for HiFi reads. C Percentage of reads with a quality score of Q30 and higher (dotted line). On average, 63% of HiFi reads and 57% of Duplex reads have a quality score of Q30 and higher