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. 2024 Dec 18;17:146. doi: 10.1186/s13068-024-02591-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 6 — Batch fermentations of C. thermocellum strains with wild-type glycolysis (strains LL1590 and LL1592) and PP_i-free glycolysis (strain LL1711). Fermentations were performed with 100 g/L cellobiose in MTC-7 chemically defined medium with pH maintained at 7.0 ± 0.05 by addition of 4 M potassium hydroxide. The shaded background represents different phases of fermentation: dark gray, growth coupled fermentation; medium gray, growth uncoupled fermentation; and light gray, no ethanol production phases. One representative fermentation profile is shown for each strain (n ≥ 2). Figure S5 shows a biological duplicate of this experiment. Additional File 3 (LL1590-1, LL1592-1, and LL1711-1) shows additional fermentation product data for this experiment.