Figure 5.

PMR4 contributes to Pi translocation under phosphate deficiency.

(A, B) Pi contents per root or shoot fresh weight (FW) of 6‐days‐old Arabidopsis seedlings exposed to low Pi (150 μM) or normal Pi (625 μM) for 9 days.

(C) Illustration of Pi accumulation in the indicated genotypes, referred to (Figures 4A, 5A,B).

(D) Ratio of ³²P in the shoots relative to the whole plants after pulse‐chase labeling. Seedlings grown on low and normal Pi media (50 and 625 μM, respectively) were exposed to ³²P‐labeled Pi for 10 min, and then transferred to non‐labeled Pi media for 120 min (pulse chase). The ³²P ratio of the shoots to the whole seedlings was calculated for the translocation rate of Pi. Data show means ± SD (n = 3 ~ 4) in (A, B, D). Asterisks (**P < 0.01) indicate statistically significant differences relative to WT according to Student's t‐test (A, B). Statistical significance was assessed by one‐way ANOVA and Tukey HSD test for each medium dataset (D). Different letters indicate significant differences (P < 0.05). NS, not significant.