FIGURE 4.

Endogenous total tau levels increase in secondary dendrites after PTX administration. (a) Hippocampal neuron expression of total endogenous tau (green, Tau Dako antibody) under basal control and 24 h. PTX conditions. (b) Quantification of endogenous tau intensity in secondary dendrites from (a); n = 18–19 neurons from at least 12 separate coverslip plantings of 2 independent culture preparations. ROUT outlier test (Q = 1%). D'Afostino and Pearson test (alpha = 0.05) was used to test for normality and passed; V2 and PTX, p = 0.4075 and p = 0.0848, respectively. Data are means ± SEM. ****p < 0.0001, two‐tailed Student's t‐test. Scale bars represent 20 μm.