FIGURE 5.

Effects of C‐terminal tau phosphosite mutations on excitatory postsynapses. (a) Schematic of the hyperexcitation‐responsive amino acid residues used for mutagenesis (highlighted with yellow box underneath representative PTX stimulation phosphomap). (b) Hippocampal neuron localization and expression of Flag‐tau control and mutant constructs as indicated at top. Flag‐tau constructs were transfected into hippocampal neurons and co‐stained for excitatory synaptic marker PSD‐95 (green) and Flag tag (red). (c) Quantification of PSD‐95 integrated intensity from (b); n = 9–13 transfected neurons from at least 8 separate coverslip plantings per 2 independent culture preparations. Data are means ± SEM. **p < 0.001, *p < 0.01, ns = not significant; D'Afostino and Pearson test (alpha = 0.05) was used to test for normality (passed; for V2, BA, and BD, p = 0.5061, 0.3728, and 0.07327, respectively); ROUT outlier test (Q = 1%). ANOVA (F(2, 29) = 7.263, p = 0.0028) with Tukey's post‐hoc test. Scale bar represents 20 μm.