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. 2024 Dec 19;22:770. doi: 10.1186/s12951-024-03032-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Expression of CD47 and common markers on EV Surfaces. (A) Schematic diagram depicting ExoView 200-based EV biological characterization. (B) Representative images demonstrating co-localization of CD81 and CD9 on individual EVs. An enlarged image is displayed in the top right corner to highlight details. Scale bar = 20 μm. (C&D) Quantification of CD9⁺ and CD81⁺ EVs from CD81 or CD9 capture spots. (E-J) Aldehyde/sulfate latex beads-based flow cytometric histograms detail CD81, CD63, and CD47 expression on individual EVs (n = 5). Statistical evaluations were conducted using one-way ANOVA. Results were presented as mean ± SEM (*** P < 0.001)