Fig. 7.

“Engage & Evasion” strategy boosts endothelial proangiogenic efficacy by MPS evasion. (A) Schematic diagram illustrating “Engage & Evasion” strategy in vitro. Initially, RAW264.7 macrophages were exposed to DV for 4 h to mimic MPS phagocytosis. Subsequently, therapeutic MV⁴⁷ was administered for another 4 h. The resultant conditional medium was then applied to HUVEC endothelial cells. (B-D) Flow cytometric histograms alongside quantification for DiD signals in RAW264.7 (n = 5). (E-G) Flow cytometric histograms alongside quantification for DiD signals in HUVECs (n = 5). (H&I) Matrigel-based tube formation assay and quantification for tube length and number. Scale bar = 200 μm. (J&K) Representative images from scratch wound healing assay, alongside quantification for closure ratio (n = 4). Scale bar = 100 μm. (L&M) Representative EdU incorporation images and percentage of EdU⁺ cells. Scale bar = 100 μm. Statistical evaluations were conducted using one-way ANOVA. Results were presented as mean ± SEM (* P < 0.05; ** P < 0.01; *** P < 0.001)