Table 3.
PI(4,5)-P2-binding of mutants assayed by gel filtration
| [PI(4,5)-P2]50% | |
| Human profilin I | 29 |
| R74A | 39 |
| R74E | 64 |
| R88A | 62 |
| R135D | 45 |
| R136D | 159 |
| S56E/R74A | 44 |
| S56E/R74E | 50 |
| S56E/R88E | 82 |
| R74E/R88E | 85 |
| R135A/R136A | 52 |
| R88A/R136D | 260 |
| R88E/R136D | 679 |
| W3A | 30 |
| rat profilin IIa | 155 |
| W3A | 27 |
PI(4,5)-P2-binding of mutants assayed by gel filtration (see Figure 5). Listed are the PI(4,5)-P2 concentrations where 50% of profilin is bound to the PI(4,5)-P2 micelles. In case of R88E/R136D, C50% was not reached and the listed value is an extrapolated value